Non-ribosomal Propeptide Precursor in Nocardicin A Biosynthesis Predicted from Adenylation Domain Specificity Dependent on the MbtH Family Protein NocI
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NRPSpredictor2—a web server for predicting NRPS adenylation domain specificity
The products of many bacterial non-ribosomal peptide synthetases (NRPS) are highly important secondary metabolites, including vancomycin and other antibiotics. The ability to predict substrate specificity of newly detected NRPS Adenylation (A-) domains by genome sequencing efforts is of great importance to identify and annotate new gene clusters that produce secondary metabolites. Prediction of...
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Background: L-tyrosine adenylation is a key step in aminocoumarin antibiotic and vancomycin biosynthesis. Results: Several, but not all tyrosine-adenylating enzymes require MbtH-like proteins for activity, forming heterotetrameric complexes. A single L→M mutation creates an MbtH-independent enzyme. Conclusion and Significance: MbtH-like proteins are essential tools in the combinatorial biosynth...
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Structure of the adenylation domain DltA 1 CRYSTAL STRUCTURE OF DLTA: IMPLICATIONS FOR THE REACTION MECHANISM OF NON-RIBOSOMAL PEPTIDE SYNTHETASE (NRPS) ADENYLATION DOMAINS* Huma Yonus, Piotr Neumann, Stephan Zimmermann, Jürgen J. May, Mohamed A. Marahiel and Milton T. Stubbs From the Institut für Biochemie und Biotechnologie, Martin-Luther-Universität HalleWittenberg, Kurt-Mothes-Straße 3, D-0...
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Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a rapidly growing class of natural products. RiPP precursor peptides can undergo extensive enzymatic tailoring to yield structurally and functionally diverse products, and their biosynthetic logic makes them attractive bioengineering targets. Recent work suggests that unrelated RiPP-modifying enzymes contain structur...
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It is now well established that the biosynthesis of the cyclic peptides gramicidin S and tyrocidine [2] are synthesized independently of ribosomes and it has been suggested that the linear gramicidins are synthesized by a similar mechanism [3] . However, since no cell free system for the study of the synthesis of this peptide is yet available, it was considered of interest to investigate in who...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2013
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja307710d